![]() The method of claim 21, wherein the tissue of interest is lung tissue.Ģ3. The method of claim 1, wherein the window frame and window are chronically implanted in the subject.Ģ2. The method of claim 1, wherein the tissue of interest is liver, lung, kidney, pancreas, colon, intestines, stomach, esophagus, ovary, uterus, bladder, prostate, bone marrow, brain, mammary gland, lymph node or salivary gland tissue.Ģ1. The method of claim 1, wherein cells or other structures in the tissue of interest are labeled using genetically encoded fluorescent proteins or injectable fluorescent dyes.Ģ0. The method of claim 1, wherein in step d), the images are obtained in a specific order so that the spatial relationships of the images is maintained and the overall architecture of the tissue is reconstructed.ġ9. The method of claim 1, wherein the tissue is imaged to a depth of at least 100 μm with a step size of 5 μm between each z-slice.ġ8. The method claim 15, wherein the lens is 25× 1.05 NA.ġ7. The method of claim 1, wherein images are obtained using a low-magnification, high-numerical aperture, long-working distance objective lens.ġ6. The method of claim 1, wherein the window frame comprises a beveled edge that rests against the tissue of interest, a groove into which a skin flap or ribcage fits, and a recess for the window.ġ5. The method of claim 1, wherein fiducial markers on the window frame serve as navigational reference points and, along with the xy stage origination, form a set of vectors from which a coordinate transformation connecting repeated imaging sessions can be derived.ġ4. The method of claim 1, wherein the window frame comprises a plurality of fiducial markers.ġ3. The method of claim 1, wherein the fixturing plate is placed between the skin of the subject and the outside lip of the window frame to stabilize the tissue of interest, wherein the fixturing plate has a recess for holding the window frame, and wherein the fixturing plate is secured to the microscope stage.ġ2. The method of claim 1, wherein the window frame is made of metal and/or plastic.ġ1. ![]() The method of claim 7, wherein the tissue is a mammary fat-pad, lymph node or salivary gland.ġ0. The method of claim 7, wherein the rigid backing is made of one or more of rubber, plastic and metal.ĩ. The method of claim 1, wherein additional stabilization of the tissue of interest is provided by affixing a rigid backing to a side of the tissue of interest opposite to the objective lens of the microscope.Ĩ. The method of claim 1, comprising attaching the window frame to the tissue of interest and applying saline within the window frame before the window is affixed to the window frame to cover the tissue of interest with saline.ħ. The method of claim 1, wherein the window frame is attached to the subject using adhesive.Ħ. ![]() The method of claim 1, wherein the fixturing plate is made of metal and/or plastic.ĥ. The method of claim 1, wherein the fixturing plate comprises portions defining a slotted recess that holds the window frame.Ĥ. The method of claim 1, wherein the fixturing plate comprises portions defining a circular hole for the window and a circular recess that holds the window frame.ģ. A method of high-resolution intravital imaging of a tissue of interest in a subject using a microscope comprising: a) exposing the tissue of interest in the subject, b) attaching a window frame holding a transparent window to the subject so that the tissue of interest is viewable through the window, c) attaching the window frame to a microscope stage with a window fixturing plate that is movable relative to the optical axis of the microscope in at least x- and y-directions, and d) acquiring through the window high-magnification, high-resolution overlapping images of the tissue of interest in a mosaic pattern and stitching the images together to produce a low-magnification, high-resolution overview image of the tissue of interest that preserves subcellular resolution, thereby obtaining high-resolution intravital images of a tissue of interest in a subject.Ģ.
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